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Cloning and Expression
Enzymes
BfaI
Supplier Images
BfaI
BfaI from New England Biolabs
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Citations:
(12)
Supplier Page
Supplier Page from
New England Biolabs for
BfaI
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BfaI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0568L
Quantity
2500 units
Concentration
5 u/µL
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
C/TAG
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (12)
(1)
Antagonistic action of GPS2 and KDM1A at enhancers governs alternative macrophage activation by interleukin 4
Nucleic Acids Research
February 22, 2023
Zhiqiang Huang, Astradeni Efthymiadou, Ning Liang, Rongrong Fan, Eckardt Treuter
DNA was purified by phenol-chloroform buffer and further digested with the second enzyme
BfaI
(
NEB
,
R0568L
) at 37°C overnight. The second restriction enzyme was inactivated by incubating the mix for 20 min at
(2)
GSK3 inhibition rescues growth and telomere dysfunction in dyskeratosis congenita iPSC-derived type II alveolar epithelial cells
eLife
May 13, 2022
Fernandez RJ, Gardner ZJG, Slovik KJ, Liberti DC, Estep KN, Yang W, Chen Q, Santini GT, Perez JV, Root S,
et al
.
FSP995H-INCL, "Peptide, recombinant protein",
CviAII
,
NEB
,CAT#
R0640L
, "Peptide, recombinant protein",
BfaI
,
NEB
,CAT#
R0568L
, "Peptide, recombinant protein",
MseI
,
NEB
,CAT#
R0525L
, "Peptide, recombinant protein",
NdeI
,
NEB
,CAT#
(3)
Quantitative spatial and temporal assessment of regulatory element activity in zebrafish
eLife
November 19, 2021
Shipra Bhatia, Dirk Jan Kleinjan, Kirsty Uttley, Anita Mann, Nefeli Dellepiane, Wendy A Bickmore
#68310, Recombinant DNA reagent,
NlaIII
(enzyme),
NEB
,
R0125S
, Recombinant DNA reagent,
BfaI
(enzyme),
NEB
,
R0568S
, Recombinant DNA reagent,
DpnII
(enzyme),
NEB
,
R0543S
, "Chemical compound, drug",PTU (1-phenyl2-thio-urea),Sigma-Aldrich,S515388, "Chemical
(4)
Primary Cilia Formation Does Not Rely on WNT/β-Catenin Signaling
Frontiers in cell and developmental biology
January 1, 2021
Bernatik O, Paclikova P, Kotrbova A, Bryja V, Cajanek L.
forward: cagtgccaggggaagaag, and AXIN2 reversed: gtcttggtggcaggcttc.
PCR
products were cut by
BfaI
(
R0568S
,
NEB
) in case of LRP5 KO and
Hpy188III
(
R0622S
,
NEB
) for AXIN2 KO
screening
, respectively. Successful disruption
(5)
Her9/Hes4 is required for retinal photoreceptor development, maintenance, and survival
Scientific Reports
December 1, 2020
Cagney E. Coomer, Stephen G. Wilson, Kayla F. Titialii-Torres, Jessica D. Bills, Laura A. Krueger, Rebecca A. Petersen, Evelyn M. Turnbaugh, Eden L. Janesch, Ann C. Morris
carrying a 1 bp insertion was identified by RFLP due to the introduction of a
BfaI
restriction site (
NEB
:
R0568S
). Genomic DNA from whole embryos or from tail clips was extracted and amplified by
PCR
as described
(6)
Yellow sea mediated segregation between North East Asian Dryophytes species
PLOS ONE
June 24, 2020
Amaël Borzée et al.
Quick-DNA Miniprep™ Plus Kit (Zymo #D4069), then 500 ng of genomic DNA was digested by 0.5 U of
BfaI
(
NEB
, Cat#
R0568S
) and
MboI
(
NEB
, Cat#
R0147S
) with 10X
CutSmart buffer
(
NEB
, Cat#
B7204S
) at 37˚C for 1 hour. We chose
(7)
Tet inactivation disrupts YY1 binding and long-range chromatin interactions during embryonic heart development
Nature Communications
December 1, 2019
Shaohai Fang et al.
the nuclei. For the Mpdu1 loci,
DpnII
(
NEB
R0543) was used for the primary digestion and
BfaI
(
NEB
R0568S
) was used for the secondary digestion.
PCR
was performed using the Roche Expand Long Template polymerase
(8)
YY1 Is a Structural Regulator of Enhancer-Promoter Loops
Cell
December 14, 2017
Abraham S. Weintraub et al.
the second digestion was performed overnight in 450 μL with 50 units of restriction enzyme.
BfaI
(
NEB
R0568S
) was used for Etv4 and
CviQI
(
NEB
R0639S
) was used for Raf1. Samples were phenol-chloroform extracted
(9)
Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons
Nature Protocols
April 1, 2014
Zoltán Ivics et al.
(
New England Biolabs
, cat no. R0179S) •
BfaI
restriction endonuclease (
New England Biolabs
, cat no.
R0568S
) •
DpnII
restriction endonuclease (
New England Biolabs
, cat no.
R0543S
) • Taq DNA polymerase, provided
(10)
Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons
Nature Protocols
April 1, 2014
Zoltán Ivics et al.
contact with skin, eyes and airways. •
BfaI
restriction endonuclease (
New England Biolabs
, cat. no.
R0568S
) •
DpnII
restriction endonuclease (
New England Biolabs
, cat no.
R0543S
) • Taq DNA polymerase, provided
(11)
Germline transgenesis in rodents by pronuclear microinjection of Sleeping Beauty transposons
Nature Protocols
April 1, 2014
Zoltán Ivics et al.
(
New England Biolabs
, cat no. R0179S) •
BfaI
restriction endonuclease (
New England Biolabs
, cat no.
R0568S
) •
DpnII
restriction endonuclease (
New England Biolabs
, cat no.
R0543S
) • Taq DNA polymerase, provided
(12)
Identification of
Sleeping Beauty
Transposon Insertions in Solid Tumors using Linker-mediated PCR
Journal of Visualized Experiments
February 1, 2013
Callie L. Janik, Timothy K. Starr
Genomic DNA from
Tumors
Name of the reagent Company Catalogue number
BfaI
New England Biolabs
R0568S
NlaIII
New England Biolabs
R0125S
MinElute 96 well plates
Qiagen
28051
QIAvac 96 Vacuum manifold
Qiagen
19504
Multi-MicroPlate
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BfaI from New England Biolabs
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